標題: A highly sensitive system for urea detection by using CdSe/ZnS core-shell quantum dots
作者: Huang, Chin-Ping
Li, Yaw-Kuen
Chen, Teng-Ming
應用化學系
Department of Applied Chemistry
關鍵字: assay;quantum dots;photoluminescence;biosensor;urea;urease
公開日期: 15-三月-2007
摘要: An original and novel assay system with urease as a catalyst and CdSe/ZnS quantum dots (QDs) as an indicator has been developed for quantitative analysis of urea. By mixing urease and QDs, the determination of urea can be performed in a quantitative manner. The detection is based on the enhancement of QD photoluminescence (PL) intensity, which is correlated to the enzymatic degradation of urea. By controlling the buffer concentration and pH, PL enhancement due to the degradation of urea is linear in the urea concentration ranging from 0.01 to 100 mM. This property makes the urease/QDs system to be a promising urea-biosensing system. The newly developed system is a superior design and possesses many advantages, including its simple preparation, low cost, no enzyme immobilization required, high flexibility, and good sensitivity. (c) 2006 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.bios.2006.09.003
http://hdl.handle.net/11536/11026
ISSN: 0956-5663
DOI: 10.1016/j.bios.2006.09.003
期刊: BIOSENSORS & BIOELECTRONICS
Volume: 22
Issue: 8
起始頁: 1835
結束頁: 1838
顯示於類別:期刊論文


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  1. 000244676300039.pdf