標題: Cytopiloyne, a novel polyacetylenic glucoside from Bidens pilosa, functions as a T helper cell modulator
作者: Chiang, Yi-Ming
Chang, Cicero Lee-Tian
Chang, Shu-Lin
Yang, Wen-Chin
Shyur, Lie-Fen
生物科技學系
Department of Biological Science and Technology
關鍵字: Bidens pilosa;polyacetylenic glucoside;T cell differentiation
公開日期: 4-Apr-2007
摘要: An extract of Bidens pilosa, an anti-diabetic Asteraceae plant, has recently been reported to modulate T cell differentiation and prevent the development of non-obese diabetes (NOD) in NOD mice. In this paper, a novel bioactive polyacetylenic glucoside, cytopiloyne (1), was identified from the Bidens pilosa extract using ex vivo T cell differentiation assays based on a bioactivity-guided fractionation and isolation procedure. Its structure was elucidated as 2 beta-D-glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne by various spectroscopic methods. Functional studies showed that cytopiloyne was able to inhibit the differentiation of naive T helper (Th0) cells into type I T helper (Th1) cells but to promote the differentiation of Th0 cells into type II T helper (Th2) cell. Accordingly, cytopiloyne also suppressed IFN-gamma expression and promoted IL-4 expression in mouse splenocytes ex vivo. These results suggest that cytopiloyne functions as a T cell modulator that may directly contribute to the ethnopharmacological effect of Bidens pilosa extract on preventing diabetes. Moreover, cytopiloyne can serve as an index compound for quality control of lot-to-lot extract preparations of Bidens pilosa. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.jep.2006.10.007
http://hdl.handle.net/11536/10930
ISSN: 0378-8741
DOI: 10.1016/j.jep.2006.10.007
期刊: JOURNAL OF ETHNOPHARMACOLOGY
Volume: 110
Issue: 3
起始頁: 532
結束頁: 538
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