標題: Fabrication of a miniature CMOS-based optical biosensor
作者: Ho, Wei-Jen
Chen, Jung-Sheng
Ker, Ming-Dou
Wu, Tung-Kung
Wu, Chung-Yu
Yang, Yuh-Shyong
Li, Yaw-Kuen
Yuan, Chiun-Jye
生物科技學系
應用化學系
電子工程學系及電子研究所
Department of Biological Science and Technology
Department of Applied Chemistry
Department of Electronics Engineering and Institute of Electronics
關鍵字: sol-gel;CMOS;optical biosensor;ormosil
公開日期: 15-Jun-2007
摘要: This work presents a novel, miniature optical biosensor by immobilizing horseradish peroxidase (HRP) or the HRP/glucose oxidase (GOx) coupled enzyme pair on a CMOS photosensing chip with a detection area of 0.5 mm x 0.5 mm. A highly transparent TEOS/PDMS Ormosil is used to encapsulate and immobilize enzymes on the surface of the photosensor. Interestingly, HRP-catalyzed luminol luminescence can be detected in real time on optical H2O2 and glucose biosensors. The minimum reaction volume of the developed optical biosensors is 10 RL. Both optical H2O2 and glucose biosensors have an optimal operation temperature and pH of 20-25 degrees C and pH 8.4, respectively. The linear dynamic range of optical H2O2 and glucose biosensors is 0.05-20 mM H2O2 and 0.5-20 mM glucose, respectively. The miniature optical glucose biosensor also exhibits good reproducibility with a relative standard deviation of 4.3%. Additionally, ascorbic acid and uric acid, two major interfering substances in the serum during electrochemical analysis, cause only slight interference with the fabricated optical glucose biosensor. In conclusion, the CMOS-photodiode-based optical biosensors proposed herein have many advantages, such as a short detection time, a small sample volume requirement, high reproducibility and wide dynamic range. (c) 2007 Published by Elsevier B.V.
URI: http://dx.doi.org/10.1016/j.bios.2006.12.031
http://hdl.handle.net/11536/10682
ISSN: 0956-5663
DOI: 10.1016/j.bios.2006.12.031
期刊: BIOSENSORS & BIOELECTRONICS
Volume: 22
Issue: 12
起始頁: 3008
結束頁: 3013
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