Title: Multifunctional micelles for cancer cell targeting, distribution imaging, and anticancer drug delivery
Authors: Huang, Chun-Kai
Lo, Chun-Liang
Chen, Hung-Hao
Hsiue, Ging-Ho
應用化學系
Department of Applied Chemistry
Issue Date: 24-Sep-2007
Abstract: Multifunctional micelles for cancer cell targeting, distribution imaging, and anticancer drug delivery were prepared from an environmentally-sensitive graft copolymer, poly(N-isopropyl acrylamide-co-methacryl acid)-g-poly(D,L-lactide) (P(NIPAAm-co-MAAc)-g-PLA), a diblock copolymer, methoxy poly(ethylene glycol)-b-Poly(D,L-lactide) (mPEG-PLA) and two functionalized diblock copolymers, galactosamine-PEG-PLA (Gal-PEG-PLA) and fluorescein isothiocyanate-PEG-PLA (FITC-PEG-PLA). Anticancer drug, free base doxorubicin (Dox) was incorporated into the inner core of multifunctional micelles by dialysis. From the drug release study, a change in pH (from pH 7.4 to 5.0) deformed the structure of the inner core from that of aggregated P(NIPAAm-co-MAAc), causing the release of a significant quantity of doxorubicin (Dox) from multifunctional micelles. Multifunctional micelles target specific tumors by an asialoglycoprotein (HepG2 cells)-Gal (multifunctional micelle) receptor-mediated tumor targeting mechanism. This mechanism then causes intracellular pH changes which induce Dox release from multifunctional micelles and that micelles have strong effects on the viability of HepG2 cells and are abolished by galactose. Confocal laser scanning microscopy (CLSM) reveals a clear distribution of multifunctional micelles. With careful design and sophisticated manipulation, polymeric micelles can be widely used in cancer diagnosis, cancer targeting, and cancer therapy simultaneously.
URI: http://dx.doi.org/10.1002/adfm.200600818
http://hdl.handle.net/11536/10320
ISSN: 1616-301X
DOI: 10.1002/adfm.200600818
Journal: ADVANCED FUNCTIONAL MATERIALS
Volume: 17
Issue: 14
Begin Page: 2291
End Page: 2297
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