標題: Reduction of the interferences of biochemicals and hematocrit ratio on the determination of whole blood glucose using multiple screen-printed carbon electrode test strips
作者: Lin, Yueh-Hui
Shen, Thomas Y.
Chang, C. Allen
生物科技學系
電控工程研究所
Department of Biological Science and Technology
Institute of Electrical and Control Engineering
關鍵字: screen-printed carbon electrodes;electrochemical biosensor;glucose;interference;hematocrit ratio;uric acid
公開日期: 1-十一月-2007
摘要: A practical approach to reduce the interferences of biochemicals and hematocrit ratio (Hct%) in the determination of whole blood glucose using multiple screen-printed carbon electrode (SPCE) test strips is described. SPCE test strips with and without glucose oxidase [i.e., GOD(+)-SPCEs and GOD(-)-SPCEs] were used and the chronoamperometric currents of test glucose solutions with various spiked uric acid concentrations and Hct% were measured. By establishing the interference relationships between glucose concentrations and uric acid concentrations as well as Hct% values and with appropriate corrections, the whole blood glucose determinations could be made to be more accurate and comparable to those determined by the reference YSI method. Specifically, the use of the Delta I value, i.e., the current difference between GOD(+)-SPCE and GOD(-)-SPCE measurements, would reduce most of the uric acid/biochemical interferences. An interpolation method was also established to correct for the glucose determinations with Hct% interferences. The Hct% corrections using the interpolation method are especially important and necessary for those blood samples with glucose concentrations higher than 110 mg dL(-1) and Hct% values lower than 35%. This approach should also be applicable to other biochemical determinations using similar electrochemical techniques.
URI: http://dx.doi.org/10.1007/s00216-007-1521-7
http://hdl.handle.net/11536/10161
ISSN: 1618-2642
DOI: 10.1007/s00216-007-1521-7
期刊: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume: 389
Issue: 5
起始頁: 1623
結束頁: 1631
顯示於類別:期刊論文


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  1. 000250537100033.pdf