標題: Direct visualization of triplex DNA molecular dynamics by fluorescence resonance energy transfer and atomic force microscopy measurements
作者: Chang, Chia-Ching
Lin, Po-Yen
Chen, Yen-Fu
Chang, Chia-Seng
Kan, Lou-Sing
生物科技學系
Department of Biological Science and Technology
公開日期: 12-Nov-2007
摘要: We have detected the dynamics of 17-mer DNA triplex dissociation mechanism at the molecular level. Fluorescence resonance energy transfer (FRET) was used as an indicator of intermolecular interaction in nanometer range, whereas atomic force microscopy (AFM) was employed to address single molecule with sub-angstrom precision. The maximum rupture force of DNA triplex was found at pH 4.65, consistent with macroscopic observations. These results indicated that the FRET together with an AFM detection system could be used to reveal the DNA triplex interaction in nanometer scale unambiguously. (c) 2007 American Institute of Physics.
URI: http://dx.doi.org/10.1063/1.2809406
http://hdl.handle.net/11536/10115
ISSN: 0003-6951
DOI: 10.1063/1.2809406
期刊: APPLIED PHYSICS LETTERS
Volume: 91
Issue: 20
結束頁: 
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