標題: Studies of surface-modified gold nanowires inside living cells
作者: Kuo, Chiung-Wen
Lai, Ain-Jung
Wei, Kung Hwa
Chen, Peilin
材料科學與工程學系
Department of Materials Science and Engineering
公開日期: 17-十二月-2007
摘要: The cytotoxicity of various surface-functionalized gold nanowires with different aspect ratios is investigated by (3-(4,5-di-me thylthiazol-2-yl)2,5-diphenyltetrazolium bromide) (MTT) assays for two cell lines, fibroblast and HeLa. It is found that functionalized gold nanowires with a diameter of 200 nm and lengths up to a few micrometers can be readily internalized by both types of cells regardless of the type of surface functionalization. However, the cytotoxicity of the gold nanowires is observed to depend on their surface modification. Serum-coated gold nanowires are the least toxic, whereas more than 50% of the cells are damaged in the presence of mercapto-acid-modified gold nanowires even at very low concentrations (10(3) nanowiresmL(-1)). Nanowires with different aspect ratios exhibit the same cytotoxicity within limits of experimental error. However, the uptake efficiency is found to be higher for shorter nanowires as compared to their longer counterparts. Therefore, we conclude that internalized nanowires with high aspect ratios are more toxic to cells than nanowires with low aspect ratios. Positively charged aminothiol-modified gold nanowires are employed to deliver both plasmid DNA and probe molecules into cells without compromising the viability of the cells. The local environment of individual nanowires within the cells is studied by monitoring the fluorescence signal from probe molecules attached to the nanowires.
URI: http://dx.doi.org/10.1002/adfm.200601133
http://hdl.handle.net/11536/10003
ISSN: 1616-301X
DOI: 10.1002/adfm.200601133
期刊: ADVANCED FUNCTIONAL MATERIALS
Volume: 17
Issue: 18
起始頁: 3707
結束頁: 3714
顯示於類別:期刊論文


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